Homeister arrived at the track tied with P.J. Cooksey at 2,137 lifetime wins but took over sole possession of second place with the 1½-length victory over Grant Me Sunshine.
The all-time leading female rider is Julie Krone, who retired with 3,704 wins.
This is Homeister's first season competing at Colonial Downs, and she is literally pulling away in the local jockey standings. The 37-year-old has collected 45 victories so far, 16 more than second-place rider Sheldon Russell.
"This is a big moment for me and another key career accomplishment that gets added to my resume," Homeister said.